Pharmaqube journal
Research Article - (2025) Volume 1, Issue 1
Evaluation of In vitro Anti Anemic Activity of abelmoschus esculentus l. Moench Fruit Extract by Using Phenylhydrazine Induced Anemia
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Received Date: Jul 07, 2025 / Accepted Date: Jul 26, 2025 / Published Date: Aug 12, 2025
Abstract
This research investigates the in vitro anti-anemic activity of Abelmoschus esculentus L. Moench (okra) fruit extracts using a phenylhydrazine-induced anemia model. Anemia, a condition marked by reduced red blood cell count or hemoglobin, is a significant global health concern, particularly affecting women and children. The study involved preparing both aqueous and hydroalcoholic extracts of okra fruit, followed by qualitative phytochemical screening, which revealed the presence of phenolic compounds, flavonoids, alkaloids, saponins, terpenoids, and steroids.
The anti-anemic potential was evaluated by assessing the extracts' ability to inhibit hemolysis in red blood cells exposed to phenylhydrazine, a compound that induces oxidative stress and mimics hemolytic anemia. Both extracts demonstrated dose-dependent inhibition of hemolysis, with the aqueous extract showing greater efficacy (IC�??�?? = 46.67 µg/mL) compared to the hydroalcoholic extract (IC�??�?? = 60 µg/mL). The standard reference, ascorbic acid, exhibited the highest activity (IC�??�?? = 33.33 µg/mL).
The results suggest that the anti-anemic effects of okra extracts are likely due to their antioxidant and hematopoietic phytoconstituents, such as flavonoids, polyphenols, and vitamin C, which protect erythrocytes from oxidative damage. These findings support the traditional use of okra in anemia management and highlight its potential as a natural therapeutic agent. Further in vivo and clinical studies are recommended to validate these effects.
Keywords
Drug Discovery, Anemia, Phenylhydrazine, Haemoglobin, Target Identification, Clinical Trials, Pharmaceutical Research, Iron deficiency, Vitamin B12 deficiency, Folate deficiency, Diet, Abelmoschus esculentus.
Citation: Manisha, R. L., Bhavani, B., Perveen, N., Sirvi, B., Bhushan, M. K., et al. (2025). Evaluation of In vitro Anti Anemic Activity of abelmoschus esculentus l. Moench Fruit Extract by Using Phenylhydrazine Induced Anemia. J pharm qube, 1(1), 1-13
Copyright: @2025 Manisha, R. L. et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.